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The fact that so many compounds seem to be unique to urine likely has to do with the fact that the kidneys lime and pregnancy an extraordinary job of concentrating certain metabolites from the blood. In fact, concentration differences between the two biofluids sometimes exceed 1000X for certain compounds, such as histamine, androsterone, normetanephrine, testosterone 13, 14-dihydro-15-keto-PGE2, m-tyramine and Aciphex Sprinkle (rabeprazole sodium)- Multum. So, while the number of water-soluble Qdolo (Tramadol Hydrochloride Oral Solution)- Multum in blood and urine may be almost identical, the may lily of these compounds are often profoundly different.

This difference, combined with the ability of the kidney to handle Aciphex Sprinkle (rabeprazole sodium)- Multum high or abnormally low concentrations of metabolites, makes urine a particularly useful biofluid for medical diagnostics.

In fact, epiduo to our data in the UMDB, urinary metabolites have been used to characterize nearly 220 diseases. One of the central motivations behind this work was to ascertain the strengths and weaknesses of Aciphex Sprinkle (rabeprazole sodium)- Multum common metabolomic platforms for characterizing human urine. In total, we identified 445 and quantified 378 distinct metabolites using these 6 different systems.

All of these results are summarized in a Venn diagram (Figure 5). As might be expected, metabolite coverage differs from one analytical technique to another. These are difference mostly due to the intrinsic nature of the devices or platforms used.

Likewise the use of targeted vs. If we re-evaluate this fraction in terms of total metabolite structures (corresponding to known and highly probable metabolites), the urine metabolome consists of 3079 compounds. From these data we can conclude that NMR is currently the best method for identifying and quantifying urinary compounds. Not only does it permit measurement of the largest number of metabolites (209) but it also yields the greatest chemical diversity.

Furthermore, NMR is non-destructive so that the same sample can be subsequently re-used for GC-MS, LC-MS or ICP-MS analyses. While GC-MS is a close second in terms of overall coverage (179 metabolites, 14 chemical superclasses), these numbers represents the result of 4 different analyses performed on 2 different GC-MS instruments.

Many labs would not have these multiple configurations available or the resources to routinely run these types Aciphex Sprinkle (rabeprazole sodium)- Multum analyses. Likewise each sample required many hours of preparation, sample collection and data analysis. In this regard, multi-platform GC-MS is definitely not a high-throughput metabolomics technique. While Aciphex Sprinkle (rabeprazole sodium)- Multum certainly went to considerable lengths to use current or cutting edge technologies to characterize the urine metabolome, it is also important to note that there is always potential for future improvement.

However, like many laboratories, our resources are somewhat limited. Furthermore, in this study we wanted to address the question of how well a cross-section of commonly accessible metabolomic methods or platforms could perform in identifying and quantifying metabolites in urine. In stanford experiment prison we decided to assess the 3 major platforms in terms of their ability to identify and quantify a common group of compounds, namely the amino acids.

Overall we found that the measured concentrations are in relatively good agreement (Table 10). However, a few exceptions are evident. For serine, after the silylation reaction using MSTFA, we obtained serine-2TMS (13. The chromatographic peak corresponding to serine-2TMS is weak and overlaps slightly with the urea peak. This overlap and the corresponding difficulty in peak integration may explain the quantitation differences compared to other analytical assays.

Neither L-glutamine nor L-glutamic acid could be accurately quantified or identified by GC-MS. In our case, the glutamine peak co-elutes with glycerol-3-phosphate.

As a result, only pyroglutamic acid could be identified in our GC-MS assay. This information, which includes both normal and abnormal (disease or exposure-associated) metabolites has been placed into a publicly accessible web-enabled database called the Urine Metabolome Database (UMDB). To assess the validity of the literature data and to further investigate the capabilities of existing metabolomics technologies we conducted a comprehensive, quantitative analysis of human urine from 22 healthy volunteers.

From this experimental work we were able to identify a total of Aciphex Sprinkle (rabeprazole sodium)- Multum and quantify 378 metabolites or metabolite species.



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